Laboratory of Crystallographic Studies (LEC)

Laboratory of Crystallographic Studies
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Laboratory of Crystallographic Studies (LEC)

Laboratory of Crystallographic Studies

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Research in this unit


The Laboratory of Crystallographic Studies is dedicated to the study of nucleation and growth of crystals from dissolution. The laboratory uses the knowledge generated by these fundamental studies in the design and optimization of applied processes for obtaining crystals as well as for understanding the processes of crystallization, crystalline aggregation and pattern formation in natural and technological environments. The research developed in the laboratory combines fundamental and applied science with a strong dissemination and didactic component.

 

Objectives

a) Nucleation and growth under diffusive mass transport. We investigate the crystallization behaviour in the absence of convection conditions; including gels, capillaries, porous media and microgravity. By eliminating the chaotic convective component it is possible to design experimental methods (called “counter-diffusion”) where the input of material is predictable and self-regulating. Counter-diffusion methods have been patented by the laboratory and used for the development of devices (some commercial) for the crystallization of compounds both on land and in microgravity, as well as extensive know-how for the optimisation of crystal quality and size.

b) Formation of self-organized patterns in natural media. We investigate how the coupling of mass transport and precipitation in diffusion-reaction systems produce self-organized patterns of interest in natural media and technological processes. These patterns include biomorphs, chemical gardens, viscous dendrification, fractal dendrites, Liesegang rings… The resulting self-organized patterns are tangible objects, fully or partially crystalline, that can be used as indicators of forming conditions in, for example, geological media or as nanomaterials with specific properties.

c) Biomineralization and biomimetic materials. There are two main ways through which life creates useful mineral structures: self-organization processes and crystal growth control making use of additives or organic substrates. In the laboratory we investigated both routes for the understanding of processes leading to the formation of biomaterials (e.g. fish otoliths), the implementation of these processes in the laboratory to obtain biomimetic materials (e.g. self-organized apatite ceramics) and the understanding of mineral morphogenetic processes potentially involved in the origin of life or in the detection of primitive life on earth (e.g. biomorphs).

d) Crystallography of pharmaceuticals compounds and biomacromolecules. The experience in crystallization by counter-diffusion techniques allows the laboratory to obtain quality crystals and optimal size for its study by X-ray diffraction both in the laboratory and in large synchrotron facilities. This advantage is specially advantageous in structural biology and pharmacology studies that require the structural analysis of difficult-to-crystallize compounds. The experience in sample characterization by X-ray diffraction is, at the same time, essential for the identification of structural and textural properties and of crystal defects produced in the other lines of research.

e) Provision of added value services in crystallization and crystallography. The Laboratory has coordinated the creation of an integrated research platform and services in Crystallization and Crystallography (“The Crystalization Factory” Consolider/Ingenio-2010) with the following objectives: generating practical know-how for the science of crystallization and crystallography, create added value for companies and research groups that require technological solutions in these fields, and promote the training of scientific and technical personnel to ensure a leadership position in crystallization and crystallography.

Staff

PhD Thesis

Title Type Date Authors University
Cristalización de proteínas en hidrogeles péptidos diseñados ad hoc para proteger los cristales frente al daño por radiación-X Trabajo Fin de Máster 12/07/2016 Guillermo Escolano Casado UNED
Síntesis y caracterización de cristales de lipasas entrecruzados. Aplicaciones en procesos de catálisis Trabajo Fin de Máster 11/07/2016 Raquel Fernández Penas FUNDACION GENERAL DE LA UNED (FUNED)
Calcium carbonate bio-precipitation in gelling environments via counterdiffusion Tesis Doctoral 30/09/2014 María Sancho Tomás UNIVERSIDAD INTERNACIONAL MENENDEZ Y PELAYO
Nucleation in small volumes and confinement Tesis Doctoral 28/10/2013 Isaac Rodríguez Ruiz UNIVERSIDAD DE GRANADA
Production, characterization and crystallization of the prokaryotic membrane coat protein 4978: evolutionary implications on the eukaryogenesis process Tesis Doctoral 19/07/2012 Mª Teresa Conejero Muriel UIMP-CSIC
New approaches to the kinetics of unsteady nucleation Tesis Doctoral 12/08/2011 Miguel Ángel Durán Olivencia UNIVERSIDAD INTERNACIONAL MENENDEZ Y PELAYO
Identificación de fases cristalinas de precipitación de sulfato de calcio en medios gelificados a través de la difracción de rayos x Tesis Doctoral 21/07/2010 Fabián Ricardo Rivera García UNIVERSIDAD INTERNACIONAL MENENDEZ Y PELAYO
Protein crystallisation in chromatography gel matrices Tesis Doctoral 23/06/2010 Luis Antonio Gonzalez Ramirez UNIVERSIDAD INTERNACIONAL MENENDEZ Y PELAYO

Projects

  • 2021
    • TEST DE ATRACCIÓN CRISTALINA (MONOS)

      Reference: 202130E054
      Researcher(s): Juan Manuel García Ruiz
      Date: 1 de September del 2021 - 1 de September del 2022
      Funding agency: CSIC
    • EL PAPEL DE LA SÍLICE EN QUÍMICA PREBIÓTICA Y AUOTOORGANIZACION MINERAL

      Reference: 951372
      Researcher(s): Juan Manuel García Ruiz
      Date: 1 de September del 2021 - 31 de August del 2023
      Funding agency: CSIC
    • APLICACIONES BIOTECNOLÓGICAS DE CRISTALES PROTEICOS

      Reference: PID2020-116261GB-I00
      Researcher(s): Jose Antonio Gavira Gallardo
      Date: 1 de September del 2021 - 31 de August del 2024
      Funding agency: Ministerio
    • COLABORACIÓN CIENTÍFICA ENTRE EL LEC (IACT), EL INSTITUTO WANGARI MAATHAI PARA LA PAZ Y EL MEDIO AMBIENTE DE LA UNIVERSIDAD DE NAIROBI EN KENIA Y LA UNIVERSIDAD DE BAHIR DAR DE ETIOPÍA

      Reference: KET202101
      Researcher(s): Juan Manuel García Ruiz
      Date: 1 de July del 2021 - 31 de December del 2021
      Funding agency: CSIC
    • Sistemas Farmacéuticos Multicomponente: una estrategia para superar limitaciones farmacológicas en la sociedad Andaluza

      Reference: B-FQM-478-UGR20
      Researcher(s): Duane Choquesillo Lazarte, Alicia Domínguez Martín
      Date: 1 de July del 2021 - 30 de June del 2023
      Funding agency: Junta de Andalucía
  • 2020
    • MATERIALES AVANZADOS USANDO CARBONATO CÁLCICO BIOGÉNICO DE RESIDUOS DE CONCHAS MARINAS

      Reference: PCI2020-112108
      Researcher(s): Jaime Gómez Morales
      Date: 1 de September del 2020 - 31 de August del 2023
      Funding agency: Ministerio
    • MEJORA DE LOS SERVICIOS CIENTÍFICO-TÉCNICOS DE LA FACTORÍA DE CRISTALIZACIÓN DEL INSTITUTO ANDALUZ DE CIENCIAS DE LA TIERRA DE GRANADA

      Reference: IE17-5683
      Researcher(s): Juan Manuel García Ruiz
      Date: 1 de January del 2020 - 31 de December del 2021
      Funding agency: Junta de Andalucía
    • AUTOORGANIZACIÓN MINERAL: MATERIALES AUTOENSAMBLADOS DE SÍLICE/CARBONATO

      Reference: P18-FR-5008
      Researcher(s): Fermín Otálora Muñoz,Juan Manuel García Ruiz
      Date: 1 de January del 2020 - 31 de December del 2022
      Funding agency: Junta de Andalucía
    • FACTORÍA DE CRISTALIZACIÓN: RED TEMÁTICA PARA EL AVANCE DE LA CRISTALOGRAFÍA APLICADA

      Reference: RED2018-102574-T
      Researcher(s): Jaime Gómez Morales
      Date: 1 de January del 2020 - 31 de December del 2022
      Funding agency: Ministerio
  • 2019
    • FIESTA DE LA CRISTALOGRAFÍA.

      Reference: FCT-18-1349
      Researcher(s): Juan Manuel García Ruiz
      Date: 1 de September del 2019 - 30 de June del 2020
      Funding agency: Ministerio
    • LIGHT ASSISTED-CRYSTALLIZATION T

      Reference: H2020-ERC-POC/0525-(LACRYS)
      Researcher(s): Juan Manuel García Ruiz
      Date: 1 de March del 2019 - 31 de August del 2020
      Funding agency: Unión Europea
    • ANDAMIOS BIOHÍBRIDOS ASOCIADOS CON APATITOS NANOCRISTALINOS COCRISTALES FARMACÉUTICOS PARA INGENIERÍA DE TEJIDOS

      Reference: PGC2018-102047-B-100
      Researcher(s): Jaime Gómez Morales,Duane Choquesillo Lazarte
      Date: 1 de January del 2019 - 31 de December del 2021
      Funding agency: Ministerio
  • 2018
    • BIOSENSORES UNIVERSALES

      Reference: BIO2017-91210-EXP
      Researcher(s): Jose Antonio Gavira Gallardo
      Date: 1 de November del 2018 - 31 de October del 2020
      Funding agency: Ministerio
  • 2015
    • RED DE CRISTALOGRAFÍA Y CRISTALIZACIÓN “FACTORÍA DE CRISTALIZACIÓN”

      Reference: FIS2015-71928-REDC
      Researcher(s): Jaime Gómez Morales
      Date: 1 de December del 2015 - 30 de November del 2018
      Funding agency: Ministerio
    • CONSOLIDACIÓN DE LA FACTORÍA COMO PLATAFORMA DE SERVICIOS AVANZADOS EN CRISTALIZACIÓN Y CRISTALOGRAFÍA

      Reference: 201530E011
      Researcher(s): Juan Manuel García Ruiz
      Date: 1 de January del 2015 - 30 de September del 2016
      Funding agency: Ministerio
  • 2011
    • PRODUCCIÓN Y CARACTERIZACIÓN DE CRISTALES DE PROTEÍNAS REFORZADOS Y ENTRECRUZADOS CON APLICACIONES BIOTECNOLÓGICAS

      Reference: BIO2010-16800
      Researcher(s): Jose Antonio Gavira Gallardo
      Date: 1 de January del 2011 - 31 de March del 2014
      Funding agency: Ministerio
  • 2006
    • FACTORÍA DE CRISTALIZACIÓN

      Reference: CSD2006-00015
      Researcher(s): Jaime Gómez Morales
      Date: 7 de December del 2006 - 6 de December del 2014
      Funding agency: Ministerio

Publications

Authors Year Title Magazine, volume, pages.
Lei Gan, Arunraj Chidambaram, Pol G. Fonquernie, Mark E. Light, Duane Choquesillo-Lazarte, Hongliang Huang, Eduardo Solano, Julio Fraile, Clara Viñas, Francesc Teixidor, Jorge A. R. Navarro, Kyriakos C. Stylianou, José G. Planas. 2021 A Highly Water-Stable meta-Carborane-Based Copper Metal–Organic Framework for Efficient High-Temperature Butanol Separation Journal of the American Chemical Society, 142, 8299-8311
Miguel A. Matilla, David Martín-Mora, Jose A. Gavira, Tino Krell. 2021 Pseudomonas aeruginosa as a Model To Study Chemosensory Pathway Signaling Microbiology and Molecular Biology Reviews, 85,
Raquel Fernández-Penas, Cristóbal Verdugo-Escamilla, Sergio Martínez-Rodríguez, José A. Gavira. 2021 Production of Cross-Linked Lipase Crystals at a Preparative Scale Crystal Growth & Design, 21, 1698-1707
Francisco Javier Acebedo-Martínez, Carolina Alarcón-Payer, Antonio Frontera, Rafael Barbas, Rafel Prohens, Milena Di Crisci, Alicia Domínguez-Martín, Jaime Gómez-Morales, Duane Choquesillo-Lazarte. 2021 Novel Polymorphic Cocrystals of the Non-Steroidal Anti-Inflammatory Drug Niflumic Acid: Expanding the Pharmaceutical Landscape Pharmaceutics, 13, 2140
Rogeria N. Costa, Ana L. Reviglio, Sana Siedler, Simone G. Cardoso, Yamila G. Linck, Gustavo A. Monti, Alexandre M. G. Carvalho, Jackson A. L. C. Resende, Marcelo H. C. Chaves, Helvécio V. A. Rocha, Duane Choquesillo-Lazarte, Lourdes Infantes, Silvia L. Cuffini. 2021 New Multicomponent Forms of the Antiretroviral Nevirapine with Improved Dissolution Performance Crystal Growth & Design, 20, 688-698
Francisco Javier Acebedo-Martínez, Carolina Alarcón-Payer, Lucía Rodríguez-Domingo, Alicia Domínguez-Martín, Jaime Gómez-Morales, Duane Choquesillo-Lazarte. 2021 Furosemide/Non-Steroidal Anti-Inflammatory Drug–Drug Pharmaceutical Solids: Novel Opportunities in Drug Formulation Crystals, 11, 1339
María Eugenia García-Rubiño, Antonio Matilla-Hernández, Antonio Frontera, Luis Lezama, Juan Niclós-Gutiérrez, Duane Choquesillo-Lazarte. 2021 Dicopper(II)-EDTA Chelate as a Bicephalic Receptor Model for a Synthetic Adenine Nucleoside Pharmaceuticals, 14, 426
Cristóbal Verdugo-Escamilla, Carolina Alarcón-Payer, Antonio Frontera, Francisco Javier Acebedo-Martínez, Alicia Domínguez-Martín, Jaime Gómez-Morales, Duane Choquesillo-Lazarte. 2020 Interconvertible Hydrochlorothiazide–Caffeine Multicomponent Pharmaceutical Materials: A Solvent Issue Crystals, 10, 1088
Arturo Navas, Fatin Jannus, Belén Fernández, Javier Cepeda, Marta Medina O’Donnell, Luis Díaz-Ruiz, Cristina Sánchez-González, Juan Llopis, José M. Seco, E. Rufino-Palomares, José Antonio Lupiáñez, Santiago Gómez-Ruiz, José Luis Quiles, Maurizio Battino, Duane Choquesillo-Lazarte, Ana Belén Ruiz-Muelle, Ignacio Fernández, Fernando Reyes-Zurita, Antonio Rodríguez-Diéguez. 2020 Designing Single-Molecule Magnets as Drugs with Dual Anti-Inflammatory and Anti-Diabetic Effects International Journal of Molecular Sciences, 21, 3146
Maria Vassaki, Konstantinos E. Papathanasiou, Chrystalleni Hadjicharalambous, Daphne Chandrinou, Petri Turhanen, Duane Choquesillo-Lazarte, Konstantinos D. Demadis. 2020 Self-sacrificial MOFs for ultra-long controlled release of bisphosphonate anti-osteoporotic drugs Chemical Communications, 56, 5166-5169
Rogeria Nunes Costa, Duane Choquesillo-Lazarte, Silvia Lucía Cuffini, Elna Pidcock, Lourdes Infantes. 2020 Optimization and comparison of statistical tools for the prediction of multicomponent forms of a molecule: the antiretroviral nevirapine as a case study CrystEngComm, 22, 7460-7474
Inmaculada Pérez-Toro, Alicia Domínguez-Martín, Duane Choquesillo-Lazarte, Josefa M. González-Pérez, Alfonso Castiñeiras, Juan Niclós-Gutiérrez. 2018 Highest Reported Denticity of a Synthetic Nucleoside in the Unprecedented Tetradentate Mode of Acyclovir Crystal Growth & Design, 18, 4282-4286
Duane Choquesillo-Lazarte, Vinko Nemec, Dominik Cinčić. 2017 Halogen bonded cocrystals of active pharmaceutical ingredients: pyrazinamide, lidocaine and pentoxifylline in combination with haloperfluorinated compounds CrystEngComm, 19, 5293-5299
José A. Fernandes, Mariana Sardo, Luís Mafra, Duane Choquesillo-Lazarte, Norberto Masciocchi. 2015 X-ray and NMR Crystallography Studies of Novel Theophylline Cocrystals Prepared by Liquid Assisted Grinding Crystal Growth & Design, 15, 3674-3683

Knowledge transfer

  • 2013
    • Sistemas optofluídicos y su aplicación en estudios de cristalización de moléculas pequeñas

      Date: 25/10/2013
      Reference: 201331570
      Institution: CSIC
      Author: Isaac Rodríguez-Ruiz, Andreu Llobera Adán, Juan Manuel García Ruiz, Jaime Gómez Morales
  • 2014
    • Sistema optofluídico para reacciones biocatalíticas con cristales de enzimas entrecruzados

      Date: 22/01/2014
      Reference: ES 2 541 623 A1
      Institution: CSIC
      Author: Isaac Rodríguez-Ruiz, José Antonio Gavira Gallardo, Andreu Llobera Adán, Juan Manuel García Ruiz, Jaime Gómez Morales
    • Procedimiento de obtención de nanopartículas de fosfato de calcio amorfo recubiertas de citrato y dopadas con flúor

      Date: 21/07/2014
      Reference: 201431091
      Institution: CSIC (50%), CNR (50%)
      Author: José Manuel Delgado López, Jaime Gómez Morales, Raquel Fernández Penas, Michele Iafisco, Anna Tampieri, Silvia Panseri
  • 2016
  • 2006
    • Through-Put Crystallization Cassette for the Growth and Optimization of Macromolecular Crystals

      Date: 10/10/2006
      Reference: 7.118.626
      Institution: MSFC (MFS-31948)
      Author: Ng, J.D., García-Ruiz, J.M., Gavira-Gallardo, J.A., Well M., Greg J.
  • 2016
    • Pharmaceutically active protein crystals grown in-situ within a hydrogel

      Date: 05/05/2016
      Reference: P201630584
      Institution: UGR-CSIC
      Naturaleza: Patente nacional
      Author: Luis Alvarez de Cienfuegos, José A. Gavira, Juan J Díaz-Mochón, Mayte Conejero-Muriel, Rafael Contreras-Montoya
  • 2010
    • Enantiómeros de derivados benzoheteroepínicos y su uso como agentes anticancerígenos

      Date: 23/03/2010
      Reference: 201030415
      Institution: CSIC
      Author: Juan Manuel García Ruiz, Duane Choquesillo Lazarte, Ana Isabel Jiménez Sanz, Carlos Cativiela Marín